A conserved sequence signature is essential for robust plant miRNA biogenesis
نویسندگان
چکیده
منابع مشابه
Reconstituting plant miRNA biogenesis.
T he genomes of higher eukaryotes encode not only proteins but also diverse noncoding RNAs, particularly small (20to 30-nt) regulatory RNAs (1–3). The small RNAs include microRNAs (miRNAs), siRNAs, and piwi-interacting RNAs (piRNAs) (4, 5). These small RNAs repress gene expression at the transcriptional or posttranscriptional levels and have critical functions in genome defense, growth, develop...
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Plant MIR genes are independent transcription units that encode long primary miRNA precursors, which usually contain introns. For two miRNA genes, MIR163 and MIR161, we show that introns are crucial for the accumulation of proper levels of mature miRNA. Removal of the intron in both cases led to a drop-off in the level of mature miRNAs. We demonstrate that the stimulating effects of the intron ...
متن کاملIntrons of plant pri - miRNAs enhance miRNA biogenesis and function
As you will see, all referees acknowledge that the findings are potentially interesting and suitable for publication in EMBO reports. However, both referees 1 and 2 point out that the observed effect of introns on enhancing mature miR163 levels should be extended to other miRNAs. Both referees also mention the inconsistency between your study and the one from Voinnet's lab regarding the require...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2020
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkaa077